山东大学耳鼻喉眼学报 ›› 2017, Vol. 31 ›› Issue (3): 50-54.doi: 10.6040/j.issn.1673-3770.1.2017.019

• 变应性鼻结膜炎防治进展·论著 • 上一篇    下一篇

苏皖健康人群血清免疫球蛋白E水平测定与分析

吴中飞1,怀德2,桂晓钟3,赵新1,周文成1,袁源1,田慧琴1,陆美萍1,程雷1,4,5   

  1. 1.南京医科大学第一附属医院 江苏省人民医院耳鼻咽喉科, 江苏 南京 210029;
    2.徐州医科大学附属淮安医院 淮安市第二人民医院耳鼻咽喉科, 江苏 淮安 223002;
    3.芜湖市第二人民医院耳鼻咽喉科, 安徽 芜湖 241000;
    4.南京医科大学国际变态反应研究中心, 江苏 南京 210029;
    5.江苏省临床医学研究院过敏与自身免疫性疾病研究所, 江苏 南京 210029
  • 收稿日期:2017-06-16 出版日期:2017-06-16 发布日期:2017-06-16
  • 通讯作者: 程雷. E-mail:chenglei@jsph.org.cn
  • 基金资助:
    国家自然科学基金(81200747,81300834);江苏高校优势学科建设工程(JX10231801);江苏省科教兴卫工程医学重点人才(RC2011071)

Detection and analysis of serum immunoglobulin E levels in healthy volunteers from Jiangsu and Anhui.

WU Zhongfei1, HUAI De2, GUI Xiaozhong3, ZHAO Xin1, ZHOU Wencheng1, YUAN Yuan1, TIAN Huiqin1, LU Meiping1, CHENG Lei1,4,5   

  1. 1. Department of Otorhinolaryngology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu, China;2. Department of Otorhinolaryngology, Huaian Second Peoples Hospital, Affiliated Huaian Hospital of Xuzhou Medical University, Huaian 223002, Jiangsu, China;3. Department of Otorhinolaryngology, The Second Peoples Hospital of Wuhu, Wuhu 241000, Anhui, China;4. International Centre for Allergy Research, Nanjing Medical University, Nanjing 210029, Jiangsu, China;5. The Institute of Allergy and Autoimmune Diseases, Jiangsu Clinical Medicine Research Institution, Nanjing 210029, Jiangsu, China
  • Received:2017-06-16 Online:2017-06-16 Published:2017-06-16

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摘要： 目的 通过对苏皖地区健康体检者的血清总IgE(tIgE)和特异性IgE(sIgE)水平进行测定,分析探讨其临床价值及意义。 方法 本研究采用酶联免疫捕获法和荧光免疫法,分别对苏皖地区接受健康体检的379例志愿者(男207例,女172例,17~64岁,中位数27岁)行血清tIgE和sIgE测定。血清tIgE水平≤100 kU/L为阴性,>100 kU/L为阳性;sIgE采用吸入性变应原过筛试验(Phadiatop),<0.35 kU/L(0级)为阴性,≥0.35 kU/L(1级以上)为阳性。使用SPSS 18.0统计软件分析数据,P<0.05表示差异有统计学意义,组间比较进行矫正(α=0.05/6=0.008 3)。 结果 379例健康志愿者中,血清tIgE水平介于1.48~1 903.22(中位数53.70)kU/L,男性显著高于女性(P<0.001),其中tIgE阳性和阴性者分别为129例(34.04%)和250例(65.96%);血清sIgE水平范围为0.01~45.20(中位数0.07)kU/L,男女之间无明显差异(P=0.207),其中sIgE阳性和阴性者分别为88例(23.22%)和291例(76.78%)。进一步分析,四个年龄组中血清tIgE水平在17~30岁年龄组最高(中位数62.18 kU/L, P=0.006),且男性tIgE水平在该年龄组最高(中位数80.60 kU/L, P=0.009)。血清sIgE水平在四个年龄组中无明显差异(P=0.318)。血清sIgE阳性人群中有67例(76.14%)tIgE水平>100 kU/L,而sIgE阴性人群中有62例(21.31%)tIgE水平>100 kU/L。血清tIgE阴性人群中sIgE阴性者占绝对多数(91.60%, P<0.001)。Spearman秩相关分析显示,血清tIgE与sIgE水平的相关系数为0.650,呈正相关(P<0.001)。 结论 苏皖地区健康成人的血清tIgE与sIgE水平具有较强的的相关性。低水平的血清tIgE(100 kU/L以下)有助于排除变态反应(变应原致敏),可作为一个临床参考指标。

关键词: 变应原, 免疫球蛋白E, 致敏, 成人, 健康志愿者

Abstract: Objective Serum total immunoglobulin E(tIgE)and allergen-specific immunoglobulin E(sIgE)levels were detected in a healthy population to evaluate their clinical value and significance. Methods A total of 379 healthy volunteers were recruited for this study, including 207 males and 172 females who were aged 17-64 years with a median age of 27 years old. All volunteers underwent a healthy physical examination in the Jiangsu or Anhui area. Serum levels of tIgE and sIgE were detected using a capture enzyme-linked immunosorbent assay and the enzyme-league immune method(Phadiatop), respectively. A tIgE level ≤100 kU/L was regarded negative, while a level >100 kU/L was regarded positive. For the sIgE level, Phadiatop values < 0.35 kU/L(class 0)were regarded negative, and values ≥0.35 kU/L(class ≥1)were positive. Data were analyzed using SPSS 18.0 software. A P-value less than 0.05 was 山东大学耳鼻喉眼学报31卷3期 -吴中飞,等.苏皖地区健康人群血清免疫球蛋白E水平测定与分析 \=-considered statistically significant, with a correction of α=0.05/6(P< 0.008 3)in multiple comparisons. Results Among the 379 healthy volunteers, the tIgE serum level was 1.48-1 903.22(median 53.70)kU/L, and it was significantly higher in males than in females(P<0.001). The serum level of sIgE(Phadiatop)was 0.01-45.20(median 0.07)kUA/L, with no significant difference between males and females(P=0.207). tIgE was positive and negative in 129 subjects(34.04%)and 250 subjects(65.96%), respectively, and Phadiatop was positive and negative in 88 subjects(23.22%)and 291 subjects(76.78%), respectively. The serum level of tIgE was highest in the group that was 17-30 years old(median 62.18 kU/L, P=0.006)among the four age groups, especially in males(80.60 kU/L, P=0.009). No significant difference was fund in the serum level of sIgE among the four age groups(P=0.318). There were 67 subjects(76.14%)with a tIgE level > 100 kU/L in the positive sIgE group and 62 subjects(21.31%)with a tIgE level > 100 kU/L in the negative sIgE group. Moreover, in the negative tIgE group, the majority were negative for sIgE(91.60%, P<0.001). The Spearman rank correlation coefficient between serum tIgE and sIgE levels was 0.650, which was statistically significant(positive correlation, P<0.001). Conclusion There was a positive correlation between the serum levels of tIgE and sIgE in this healthy population from the Jiangsu and Anhui areas. A lower serum level of tIgE(less than 100 kU/L)may be a reference value to exclude allergic sensitization.

Key words: Adult, Allergens, Hypersensitivity, Immunoglobulin E, Healthy volunteers

中图分类号: 

  • R765.21
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